Western blot analysis was performed on nuclear extracts (30 µg lysate) of MCF-7 (Lane 1), U-2 OS (Lane 2), HEK-293 (Lane 3) and tissue extracts of Rat brain (Lane 4). The blots were probed with Anti-MAFA Rabbit Polyclonal antibody (Product # 720262, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 48 kDa band corresponding to MAFA was observed. The molecular weight of MAFA has been reported in literature to be different from its apparent molecular weight of 37 kDa. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).